Exact(24)
Nevertheless, a need for simple, rapid, and cost-effective molecular protocols to accurately discriminate between D. immitis and D. repens still remains.
Specifically designed molecular protocols for DNA extraction and PCR amplification yielded valid sequence data for short (∼100 200 bp) regions of the mitochondrial cytochrome b (mtCYB) gene for individual euphausiids.
The two species cannot be reliably distinguished using morphological characters, but are readily identified and distinguished by simple, rapid, and inexpensive molecular protocols based on sequence variation of mitochondrial DNA (mtDNA).
Molecular protocols may however act as an important adjunct to traditional culturing.
A key challenge is to establish more widespread use of molecular protocols in developing world settings.
Contamination, however miniscule, has the potential to significantly reduce the sensitivity of the molecular protocols, if not adequately addressed.
Similar(36)
By using two sets of PCR primers, one for a universal bacterial gene (16S rRNA) and one for a species-specific gene (nuc), the molecular protocol allowed both the detection and speciation of the bacterial infection.
A new molecular protocol with appropriate sensitivity and specificity using real-time RT-PCR was developed by the Centers for Disease Control and Prevention CDCC) to identify the pandemic 2009 influenza A (H1N1) virus in human specimens.
For these replicates, the molecular protocol was carried out independently starting from the restriction-ligation reaction.
This prospective multicenter trial aimed at comparing the microbiological results obtained using this molecular protocol (easyMAG® system) and semiquantitative culture in suspected VAP.
ACC developed the molecular biology protocols, drafted the manuscript and oversaw the integration of protocol components into a single workflow.
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