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Molecular profiling was carried out using TRFLP and bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP).
Additionally, although the brain metastasis is BRAF V600E mutated, this molecular profiling was performed retroactively for research purposes.
Therapy based on molecular profiling was considered to be effective for a given patient if the ratio exceeded 1.3.
The development of tissue microarrays to enable high-throughput molecular profiling was presented by Olli Kallioniemi (National Institutes of Health, Bethesda, MA, USA).
Other possibilities for the response in this patient may be the effect of anastrazole or the development of new molecular alterations since the primary diagnosis as molecular profiling was done on primary breast tissue.
Of 86 patients for which molecular profiling was performed, a molecular target was identified in 84 (98%) patients, 66 of whom were treated according to the results of the molecular profile.
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Molecular profiling is a promising tool for classification in order to optimize prognosis prediction and treatment for an individual patient.
Real-time, high-resolution microscopy in live animals, and comprehensive genetic and molecular profiling are effective tools to interrogate diverse metastatic cancer cell phenotypes as well as the metastatic tumor microenvironment in different organs.
Molecular profiling is performed as summarized in Fig. 3.
Molecular profiling is uniquely suited for multiplex assays.
Furthermore, this molecular profiling is able to identify the subtle differences between the molecular glioma subtypes.
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