By enrichment and molecular procedures, we identified α- and β-Proteobacteria strains, classified by 16S rDNA sequences as Dechlorospirillum sp. and Dechlorosoma sp., respectively.
The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing.
We have described a fast molecular procedure (2 hours) with good sensitivity and specificity, able to quantify and differentiate strains 652 and JP2 of Aa simultaneously.
The molecular procedure mainly investigates the expression of the floral homeotic genes.
Cancer is a worldwide health problem due to the complex and progressive molecular procedure and the absence of effective diagnostic tool at cancer early stage [ 2].
Therefore, it has been recommended to combine IHC with one of the molecular techniques like pyrosequencing or high resolution melting (HRM) analysis, starting with IHC and – if negative or uninterpretable – adding the molecular procedure.
Further, the molecular procedure based upon the traditional (morphological) homology perception would require homoplasious sites to be placed in different (separate) columns in a multiple sequence alignment, as indicated in the Fig. 3 (20).
In consideration of the molecular data and nomenclatural procedure, we remove L. herculeus from the synonymy of L. terrestris, and thereby restrict L. terrestris to the cluster whose members are larger.
To maintain consistent results among countries and sample treatment, we validated standardized sampling and molecular procedures by ring test (36 ), including IAC and sample process controls (26, 27, 31 ).
We present in this work, the development of new molecular procedures for diagnosis of the aforementioned organisms, together with a molecular characterization of isolates found in southern European cats.
