The molecular probe upon interaction with different class of anions showed fluorescence turn-on behavior to detect fluoride anion (F−) selectively, in acetonitrile.
This class of in vivo molecular probe can be labeled with near-infrared (NIR) dyes for visualizing the full depth of the epithelium in small animals.
The sequence of all other probes (probes class II and III) is indicated in the corresponding figures. 1 mm of rat anti-HA (1/50; Molecular probe, Invitrogen, CA, USA) was used for the 'super-shift' experiments.
It is also apparent in the molecular probe experiments [11].
In this work, we report a new, to our knowledge, class of molecular probes based on dye-conjugated dendrimers for fluorescence imaging and single-molecule fluorescence microscopy.
Remarkably, molecular oxygen can currently not be analyzed in vivo by genetically encoded FRET-based biosensors, although these biosensors constitute one of the most widespread classes of fluorescent molecular probes used for the non-invasive quantitative analysis of intracellular compounds including Ca2+, Zn2+, Cl-, pH2O22O2, ATP, maltose, sucrose, ribose and glucose [ 25- 27].
Recently, a new class of molecules termed aptamer has emerged as excellent molecular probes for cancer diagnosis and targeting therapy [ 14, 15].
Molecular Probes, based in Eugene, Ore., had sales of $56 million last year from fluorescent markers used to label molecules in biology research.
The Invitrogen Corporation, which makes chemical kits used to analyze and clone genes, agreed to buy Molecular Probes Inc. for about $325 million to add drug-discovery products.
Table 3 summarises the PE-based radiolabelled molecular probes.
