The combined exclusion probability of the markers (0.99) used in this study for parental assignment with one known parent was calculated in COANCESTRY v1.0 (Wang 2011).
These findings are likely to be general, because uncertainty in parental assignment is a typical feature in natural populations, either because of extra-pair paternity [22] or because of limits to the reliability of molecular paternity assignment based on a finite set of markers (e.g. [23]).
The resulting pedigree structure from the parental assignment analysis is presented in Table 1.
Table 6 summarizes results from parental assignment of all the F1 progeny (N = 147) obtained by comparison of microsatellite profile between progeny and all possible parent-pairs.
Figure 3 shows the distribution of pairwise genetic distances observed between F1 full sibs, half sibs as well as unrelated individuals following parental assignment.
Besides its applicability in the selection of candidate breeders for artificial reproduction, parental assignment can be useful for the identification of individuals recaptured in the wild.
Based on this parental assignment analysis, Richard et al.
Of the 3687 fish sampled for parental assignment, 3672 were unambiguously identified to family.
Therefore, we only present classifications and statistical results obtained with parental assignment estimates of q > 0.9.
All loci were highly polymorphic, thus all potentially offering high power of parental assignment (Bernatchez and Duchesne 2000).
