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Dissolved molecular oxygen effect on the enhancement factor for each sample was examined experimentally.
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Molecular oxygen has two opposing effects: initially low oxygen concentrations limit enzyme turnover because the PHDs have a low affinity to oxygen as compared to collagen hydroxylases for example.
This decrease in the intensity is due to the fluorescence quenching effect by molecular oxygen.
The effect of molecular oxygen and oxidative stress on arginase activity was investigated by different approaches.
CAT, a highly reactive enzyme that reacts with H2O2 to form water and molecular oxygen, plays a protective antioxidant effect against the deleterious actions of lipid peroxidation.
In oxygen-sensitive He-MRI the T1-shortening effect of molecular oxygen is used to calculate the pO2 in a respective volume of interest by signal intensity changes in repeated imaging during a single breath-hold.
The molecular oxygen-enhanced MR images were acquired using an inversion recovery-preparation fast low angle shot (IR-FLASH) sequence sensitizing the paramagnetic effects of molecular oxygen using a 4.7 tesla MR system.
Ultimately, this assay relies on both the consumption of oxygen by GOx (Eq. 2) as well as the generation of a large number of initiating hydroxyl radicals (Eqs. 1– 3) to overcome the inhibitory effects of molecular oxygen.
π-Electronic edge-localized spin magnetism in defective carbon nano-onions (DCNOs) derived from detonation nanodiamonds was investigated in terms of molecular oxygen adsorption and microwave (MW) irradiation effect.
Both photochemical consumption of oxygen and microvascular shutdown can lead to depletion of molecular oxygen during PDT, limiting the biological effect (Henderson and Fingar, 1987).
Nuclear singlet relaxation from dissolved paramagnetic species may be significant.[ 14] The data on a non-degassed sample display the effect of dissolved molecular oxygen.
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