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Saliva was used as the microbiological specimen, and the samples were analyzed by molecular methods using multiplex PCR.
The primary purpose of this study was to compare the phenotypic identification of bacteria to molecular methods using 16S rDNA amplification, cloning, and sequencing in a series of sputa from CF patients.
Diagnosis of VL was based on molecular methods using Leishmania infantum polymerase chain reaction (PCR) (Taqman technology; Light Cycler target: kinetoplast mini-circle DNA) on peripheral blood and other biological samples, i.e. bone marrow, urine and kidney [ 23].
In addition, molecular methods, using the 16S rRNA and functional genes as biomarkers, could further elucidate the anammox bacteria from the phylogenetic diversity to quantitative distribution in various samples.
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Trypanosomatids are highly prevalent pathogens of Hymenoptera; however, most molecular methods used to detect them in Apis and Bombus spp. do not allow the identification of the infecting species, which then becomes expensive and time consuming.
The present report describes the molecular methods used to test for the presence of 3 previously published SNPs in M. leprae DNA extracted from ancient European skeletal remains.
The molecular methods used to detect the interactions have an inherent directionality.
Comparative analysis of the phenotypic and molecular methods used to identify Candida species.
Since the PPI itself has no directionality (it is a molecular docking phenomenon between two molecules) the disagreement comes from the molecular methods used.
The phenotyping methods used to test CoNS for methicillin resistance in the period 1998 2005 were also less sensitive than the molecular methods used today.
Of the molecular methods used, polymerase chain reaction (PCR) sequencing-based methods are powerful tools for identifying species both within [ 7- 10] and between genera [ 11- 14].
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