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Background: Prolonged therapy with ganciclovir (GCV) can result in the development of GCV-resistant strains due to mutations in the viral phosphotransferase (UL97 gene) and/or in the viral DNA polymerase (UL54 gene).Objectives: The purpose of this study was to detect by molecular methods the most prevalent UL97 mutants which confer ganciclovir-resistance in immunocompromised populations.
Among all diagnostic techniques such as culture, serology, and molecular methods, the last one is the fastest [ 7].
With established molecular methods, the isolate was found to harbor SCCmec type IV (5 ); lukS and lukF, the genes coding for the 2 subunits of the PVL toxin; and hlg, the γ-hemolysin gene (3 ).
We study here with molecular methods the evolutionary history of a highly diverse assemblage of subterranean beetles in the tribe Leptodirini (Coleoptera, Leiodidae, Cholevinae) in the mountain systems of the Western Mediterranean.
Our objective was to detect and identify, by molecular methods, the presence of Bartonella species in wild rodents from the Lao PDR (Laos), a country where epidemiological and clinical studies of zoonoses are scarce.
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Although significant associations were found between the microscopic and molecular method, the presence of outliers cannot be overlooked (Fig. 2).
KFAL conceived and designed the study, carried out the molecular methods, analyzed the data and drafted the article.
This basic knowledge will make it easy to understand the first of the two main molecular methods for the diagnosis of disease.
The application of molecular methods for the epidemiology of TB complement traditional approaches used in public health.
This result emphasizes the importance of molecular methods for the identification of antimicrobial resistance in multidrug resistant pathogenic bacteria.
In the majority of systems studied, molecular methods detect the presence of a greater number of species than culture alone.
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