From a technical point of view, most rapid tests can be done at the bedside, whereas cell culture, electron microscopy and molecular-based methods require laboratories with sophisticated equipment, experienced staff and appropriate biosafety procedures.
Currently, most MST methods rely on fecal indicator microorganisms or use molecular methods that require extensive data collection before hosts can be reliably differentiated (Green et al. 2014b; McLellan and Eren 2014; Ohad et al. 2016).
Monitoring using rapid molecular methods will require the standardization and simplification of analytical methods, as well as greater clarity about their interpretation.
Because rates of evolution and species divergence times cannot be estimated directly from molecular data, all current dating methods require that specific assumptions be made before inferring any divergence time.
Molecular methods were required to detect, characterize, and identify these fastidious and uncultivated bacteria.
Indirect methods require the prior radiosynthesis of a prosthetic group and subsequent bioconjugation to a molecular entity that has been modified for site specific reaction.
However, all these methods require some other, efficient mechanism of cellular entry, either nonspecific or based on activity of a specific molecular marker triggering cellular entry.
All of these methods require internet connection.
A molecular method, not requiring colony growth, is helpful for winemakers alike to quickly obtain information about the presence of Brettanomyces in their products.
Many molecular methods do not require use of the actual virus, which is dangerous and requires nearly the highest level of safety precautions.
