Exact(3)
Information generated from the use of culture and molecular methods performed in combination will likely continue to generate useful information about how the vaginal microbiota impacts human health.
Molecular methods performed on lymph node biopsy specimens are useful diagnostic tools, but the common single molecular approach using 16S rRNA PCR lacks sensitivity, which delayed diagnosis for this patient (3 ).
FVU samples were used because several studies have reported that molecular methods performed on urine samples can detect as many, or even more, infected patients than traditional urethral swabs, or cervical swabs or semen.
Similar(57)
Finally, screenings by the molecular method performed on DNA extracted from 56 isolates retrieved from both infected and non-infected fragments at the conclusion of inoculation experiment II in Palau (Table S3), demonstrated that results obtained by screening field isolates were consistent with screening laboratory derived isolates (Pearson's χ2 = 6.725, DF = 1, p = 0.010).
For each of the molecular biophysical methods performed below, the buffer conditions and protein concentration were identical to allow for comparative analysis.
By comparing empirical data obtained from cross-reactivity studies with the molecular modeling studies, our published data indicate that 2D molecular similarity methods perform well in predicting cross-reactivity of drugs to existing drug of abuse screening immunoassays.
Previously we have shown in several studies that 2D molecular similarity methods perform well in predicting cross-reactivity of drugs to existing drug of abuse screening immunoassays [44, 47, 49].
All molecular methods were performed using standard molecular biology techniques (Sambrook et al. 1989).
To obtain fusion proteins between full-length YopK/YopKAA and β-lactamase lacking its N-terminal signal peptide, PCR amplifications and standard molecular methods were performed.
Molecular methods were performed with the use of standard protocols (Ausubel et al. 1994).
Molecular methods were performed as described by Sambrook et al. [ 34].
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