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Mainly the strain identification relies on molecular methods like 16S rRNA sequencing, but GC-FAME can also be used.
The serological methods such as Enzyme-linked immunosorbent assay (ELISA) usually employed for diagnosis BVDV in clinical samples (Entrican et al. 1995) but at these years several molecular methods like reverse transcriptase polymerase chain reaction (RT-PCR) are use extremely to detection of BVD viral RNA for diagnostic purposes (Kim and Dobovi 2003; Gilbert et al. 1999).
Molecular methods like ribotyping and multilocus sequence typing (MLST), as well as restriction fragment length polymorphism (RFLP), pulsed-field gel electrophoresis (PFGE), and randomly amplified polymorphism DNA (RAPD) etc., have no or very low discrimination power [12] [15].
In addition, past studies only monitored microbial succession in infants with conventional molecular methods like denaturing gel gradient electrophoresis (DGGE) and SSU rRNA gene-based microarray [8], [9], [10] that have their own technical limitations.
These studies have previously been supported by quantitative analysis of the ubiquitous microbial 16S ribosomal RNA gene using traditional molecular methods like denaturing gradient gel electrophoresis (DGGE), fluorescent in situ hybridization (FISH), quantitative PCR (qPCR), or capillary sequencing using the Sanger method [7].
New molecular methods like multiplex FISH analysis could be utilised for predictive stratification of patients with EAC to select individualised treatment.
Similar(45)
"As we develop better molecular methods, people would like it if the molecular dates reconciled with the fossil record.
Although we did not confirm the identity of the mimivirus-like particles by molecular methods or electron microscopy, the nature of the light micrographs enabled close examination of the particles.
Molecular methods applied to 2,855 strains of Campylobacter-like organisms received from a surveillance network of Campylobacter infections in France identified 29 Arcobacter butzleri infections.
Of course, as molecular methods improve, and genome-wide association studies become more powerful, these potential links will become clearer.
"We are moving into a new era where we can actually use molecular methods to detect with much more sensitivity the pathogens in stools.
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