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Molecular methods, including PCR-based techniques, are available to prevent species substitutions and to authenticate food content.
Over the last 8 years he has worked extensively on macro- and micro-evolutionary studies of M. tuberculosis employing multiple molecular methods including whole-genome analysis to better understand genotype-phenotype relationships within an epidemiologic context.
A total of 114 Campylobacter isolates from 572 intestinal samples were all identified as C. coli via both classical methods and molecular methods, including 16S rDNA sequence analysis and polymerase chain reactions (PCR) using specific primer sets for the hippuricase gene and the aspartokinase gene, designed to differentiate C. coli from C. jejuni.
Mycobacteria were isolated from a collection of 90 environmental samples and identified to the species level using conventional microbiological and molecular methods including the PCR amplification of hsp65 and sequence analysis of, 16S rRNA genetic markers.
All the samples were further analyzed by using molecular methods including four multiplex RT-PCR, as previously described [2], and an adenovirus PCR (Adenovirus Consensus®, Argene).
In addition to these conventional methods, molecular methods including PCR based amplification of highly conserved sequences in the genome is also reported [6], [7].
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These novel molecular methods included a multiplex real-time PCR assay to screen for the presence of the most important tick-borne pathogens (piroplasms and anaplasmas), followed by a second species-specific multiplex real-time PCR assay for the identification of Anaplasma-positive samples.
Other molecular methods include PCR ELISA, nested and real-time PCR, which are used to detect fungal DNA in blood samples.
The molecular method includes a preprocessing liquefaction for ETA before DNA extraction.
Applicant should have demonstrated expertise in modern molecular ecology methods including quantitative PCR, DNA sequence analyses, phylogentics, as well as working with the informatics tools for microbiome and metagenomic studies.
Currently, the most frequently used methods for HPV detection are molecular biological methods, including nucleic acid hybridization-based and polymerase chain reaction (PCR -based methods.
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