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Many molecular methods do not require use of the actual virus, which is dangerous and requires nearly the highest level of safety precautions.
However, since molecular methods do not distinguish between live and death bacteria, culture confirmation is mandatory.
Since molecular methods do not require a culture step, they seem an excellent alternative for the monitoring of indoor fungal contaminations.
Tests for rapid diagnostic work involving smears and molecular methods do not require CL3 practices after the organism has been rendered non-viable.
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On the contrary, possible 7p polysomy (assessed by molecular methods) did not explain the PDGFA (or EGFR) changes in all cases.
There is also a potential risk of catheter contamination during removal, even though the clinicians work carefully and aseptically, so we can not completely exclude the possibility that some of the detected microorganisms (using cultivation and molecular methods) did not colonize the catheters in vivo.
However, phylogenies based only on a selected molecular method do not necessarily have the same topology as trees made from morphological or biochemical data [ 33, 34].
The molecular method did not identify four GP samples.
However, methods do not yet exist to observe, visualize, or model the mesoscale, the intermediate scale of 10 7 10 8 m bridging molecular and cellular biology, in full molecular detail.
Because our molecular identification method does not distinguish M. marinum from M. ulcerans, we performed a light exposure test, which identified yellow colony pigmentation typical for M. marinum.
The results are similar to those obtained for nonconjugated heparin, suggesting that the fluorescein modification and the change in average molecular weight (see Materials and Methods) did not affect the ability of heparin to release RNA from the Tau fibril cores.
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