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The molecular methods developed for differentiation of C. minus simile and C. minus verum can be used to rapidly identify these morphotypes from cultures or from infected needles, which reduces the time otherwise required for morphological identification.
Unidentified ticks were conclusively identified by molecular methods developed at UNTHSC, which used amplification of 12S rDNA (Table 1) and sequence determination (data not shown).
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The molecular method developed was validated for speciation of enterococci and was useful in assessing uncooked processed meat products as a reservoir for multi-drug resistant Enterococcus species.
So far, there was no molecular method developed for parallel detection and quantification of both waterfowl parvoviruses.
The molecular method developed in this study additionally provided identification of mixed infections as well as infections due to emergent fungal pathogens.
Molecular typing methods, developed over the past decade, are highly discriminatory in capacity and reproducibility.
Molecular-based methods developed recently can provide presumptive identification of some fungal species (e.g. C. albicans or C. glabrata) [7], or sensitive simultaneous detection and identification of fungal pathogens directly from blood culture specimens [8].
In molecular methods being developed to this end, selectively neutral genetic mutations, such as synonymous single nucleotide polymorphisms (SNPs) and extragenic tandem repeats [2] present advantages over non-neutral mutations to establish relationships between strains.
To overcome these drawbacks, other molecular methods were developed, among them multilocus variable number tandem repeat (VNTR) analysis (MLVA).
The species origin of amicronucleates was first explored when molecular methods were developed to identify species without the use of mating reactions.
The molecular biology method developed in this study aimed to produce a large quantity of data concerning the composition of human gut microbiota.
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