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There is a wide array of molecular methodologies currently available for species identification [6], but they all require that the molecular methodology used is fully validated prior to its application [8].
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Although a large number of molecular methodologies are currently available to study these bacteria, some of the techniques, such as PCR, denaturing gradient gel electrophoresis (DGGE) and thermal gradient gel electrophoresis (TGGE) [ 9, 34- 37], still require improvement, especially with regard to the sensitivity, cost and quantitative power.
These results show the need for fine-tuning of the currently available molecular methodologies for confirmation of L. monocytogenes.
Apart from the lack of molecular evidence for such a carrier, the main reasons of this controversy lie in the methodologies currently used to study anandamide cellular uptake.
Only recently has the development of molecular methodologies allowed progress [40].
These genes were then tested by other molecular methodologies.
Framework synthesis is one of several methodologies currently being developed for synthesising qualitative data [ 2].
Altogether, this work describes the first association between plant DNA and pneumonia by molecular methodology.
Is this methodology currently considered adequate by the Chinese government?
No methodology currently exists for quantifying (i.e., ng/mg) murine SerpinB2 protein expression levels.
This methodology currently forms the basis of most 2DGE redox proteomic studies (Reviewed in (10)).
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