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RPWM 2/2 earlier identified as Penicillium janthinellum but by molecular method was identified as Penicillium pinophilum.
The molecular method was carried out to characterize some non-sporulating group using the ribosomal internal transcribed spacer (ITS) sequence.
Our results indicated that the molecular method was more accurate than antigen capture ELISA that was similar to previous study (Horner et al. 1995).
Through genetic analysis the status of different species morphologically defined was confirmed and a molecular method was developed to differentiate both species.
The special feature of this new molecular method was technically rapid and convenient practically and suitable for analyzing large numbers of samples.
In conclusion, an efficient culture-independent molecular method was used to explore the diversity of xylanases in the rumen ecosystem.
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This rapid and accurate molecular method is highly promising for use in the food industry.
The higher prevalence obtained with a molecular method is expected as this method is more sensitive than culture tests.
Currently, no available molecular method is, by itself, sufficiently accurate or sensitive enough to replace methods based on blood culture.
Results from detail meat inspection, bacteriology and molecular method were recorded on Excel spread sheets for appropriate test comparison analysis.
Identification at species level by molecular methods was possible in all cases except one.
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