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Temporal Temperature Gel Electrophoresis of amplified 16S rRNA gene sequences (16S rDNA PCR-TTGE) constitutes a culture-independent molecular method used to study bacterial communities.
Positive DNA extractions from commercial cultures (Carolina, USA) of other dinoflagellates such as Amphidinium carterae and Gymnodinium spp., as well as a diatom consortium, were always negative for the targeted cp23S DNA regions, reassuring on the zooxanthellae specificity of the molecular method used.
The polymerase chain reaction (PCR) was the first molecular method used for waterfowl parvoviruses detection [ 12, 13].
The molecular method used in this study for viral detection is recognised as the most sensitive technique [ 27, 28].
Variation in this region is extensive and as a consequence toxinotyping is a frequently used molecular method used to discriminate between strains [ 34, 35].
Nevertheless, care should be taken when comparing data between studies as genetic diversity depends on numerous factors, namely life history, breeding system, growth life forms, geographical range and type of molecular method used (Powell et al. 1996; Nybom 2004).
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A significant difference was also obtained for the same 38 isolates when screened by the molecular method using PCR primers (Pearson's χ2 = 12.518, DF = 1, p<0.0001).
The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis.
Trypanosomatids are highly prevalent pathogens of Hymenoptera; however, most molecular methods used to detect them in Apis and Bombus spp. do not allow the identification of the infecting species, which then becomes expensive and time consuming.
The present report describes the molecular methods used to test for the presence of 3 previously published SNPs in M. leprae DNA extracted from ancient European skeletal remains.
The molecular methods used to detect the interactions have an inherent directionality.
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average method used
molecular approach used
empirical method used
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molecular velocity used
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molecular sieve used
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molecular docking used
molecular marker used
molecular weight used
molecular fingerprinting used
molecular model used
molecular design used
molecular structure] used
molecular system used
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