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This rapid and accurate molecular method is highly promising for use in the food industry.
The notable feature of this molecular method is that it is technically accurate, practically convenient and suitable for analyzing large numbers of samples.
Our results indicate this novel molecular method is suitable to confirm the presence and estimate the concentration of a specific LAB strain in animal feedstuffs containing high background levels of LAB.
The higher prevalence obtained with a molecular method is expected as this method is more sensitive than culture tests.
Currently, no available molecular method is, by itself, sufficiently accurate or sensitive enough to replace methods based on blood culture.
We used detection of the ipaH gene as an indicator of Shigella/EIEC infection because this molecular method is highly sensitive and specific (4, 26, 27 ).
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RPWM 2/2 earlier identified as Penicillium janthinellum but by molecular method was identified as Penicillium pinophilum.
The molecular method was carried out to characterize some non-sporulating group using the ribosomal internal transcribed spacer (ITS) sequence.
Our results indicated that the molecular method was more accurate than antigen capture ELISA that was similar to previous study (Horner et al. 1995).
In conclusion, an efficient culture-independent molecular method was used to explore the diversity of xylanases in the rumen ecosystem.
The per sample cost of the molecular method was approximately €400.
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