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A total of 24 water samples (sewage, river water and seawater) were tested both by PCR and by plaque assay, to evaluate the efficiency of the molecular method in field samples.
This variation in colonization might be due to the advanced molecular method in the previous study.
Mohaghegh et al. [ 75] used PCR for confirming negative direct stained smear, confirming the higher sensitivity of the molecular method in comparison with classical diagnosis (direct stained smear).
However, the use of this molecular method in addition to blood culture would have resulted in additional detections of clinically relevant microorganisms in some cases, which could have influenced patient outcome.
Since its development in 1983, PCR remained the most widely used molecular method in clinical microbiology, notably for detection of microorganisms in clinical specimens, until 1996 when real-time PCR (RT-PCR) was developed.
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The role of molecular methods in diagnosing infection has yet to be determined.
Together, such methodologies may constitute a novel platform for the integration of molecular methods in clinical decision-making.
Despite being a small collection of isolated Lactobacillus species, Lactobacillus brevis species was the dominant species among the identified isolates using molecular methods in this collection.
There are no studies on the detection of cytomegalovirus (CMV) DNA by molecular methods in the saliva of newborn infants in large scale screening programs.
In this review, we discuss the contribution of the main molecular methods in breast cancer research and how this information is changing our approaches to the diagnosis and management of this disease.
The English literature from 1995 to 2008 was reviewed to identify best available evidence regarding the prognostic value of isolated tumor cells and micrometastases detected with various immunohistochemistry and molecular methods in non small cell carcinoma patients.
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