The low molecular mass substrates 2′- 4-methylumbelliferyl)- N-acetylneuraminic acid (MU-NANA), 3′-sialyl- N-acetyllactosamine (3SLN) and 6′-sialyl- N-acetyllactosamine (6SLN) contained one sialic acid residue per molecule.
These findings tend to suggest that the enzyme had high affinity toward high-molecular mass substrates containing α-1,4 and α-1,6 linkages.
For tuber α-amylase, high-molecular-mass substrates containing the α-1,4-linkage were better substrates for the enzyme.
Because it was more suitable for the cleavage of high molecular mass DNA substrate the 20% DMSO concentration was consequently chosen for further experiments.
The chimeric chitinases had similar activities towards soluble substrate but higher hydrolytic activity than the native chitinase on high molecular mass insoluble substrates such as ground chitin or chitin-rich fungal cell walls.
Metabolomics is the quantitative analysis of a large number of low molecular mass metabolite, substrates or products in metabolic pathways.
This result is reasonable because some GH10 xylanases have activity on low molecular mass cellulose substrates, particularly aryl cellobiosides [ 32].
Although this assay was a simple approach to test the chaperone-like function of EhPDI, the low molecular mass of the substrate (3.4 kDa) represents a limitation of this assay, since it offers a restricted number of contact sites to form stable complexes [ 47].
Rather than simply the substrate molecular mass, our results presented here indicate that at a specific substrate concentration, it is most likely the combination of the number of substrate molecules bound to the DegP trimers forming the cage and the apparent size of the substrate what determines the functional oligomeric state reached by DegP.
The number of atoms over an area d S on the graphene and a volume d V s in the substrate are ρc d S and ρs d V s, respectively, where ρc is the homogenized carbon atom area density of graphene that is related to the equilibrium carbon carbon bond length l by and ρs is the molecular density of substrate that can be derived from the molecular mass and mass density of substrate.
Preservation of enzyme machinery in vacuoles was evident from retention of invertase activity, substrate specificity, molecular mass and subunit composition.
