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Lanes 14 17 in the same figure indicated that the primary precipitate contained mainly high molecular mass components, which were degraded to a larger extent than those in the secondary precipitate.
Humic substances (HS) are the major component of soil, water and sediment organic matter and chemically can be depicted as a collection of diverse, relatively low molecular mass components of organic molecules forming dynamic associations stabilized by hydrophobic interactions and hydrogen bonds on the nanometer scale [14].
WTF (< 1000 Da) was prepared to remove large molecular mass compounds suspected of inhibiting low molecular mass components with potential antimicrobial efficacy.
Virtually identical results were obtained with cytosols passed over a PD10 column, excluding competition for BH4 binding by low molecular mass components present in the cytosolic fractions of the human cells.
The virtually identical results obtained with desalted cytosols exclude a substrate-competitive effect of endogenous low molecular mass components of the cells, but we cannot exclude that an unknown protein present in some types of cells interferes with BH2 (but not DHF) binding.
Figure 6a right panel illustrates that in spite of the high abundance of low molecular mass components, only dimers and HMM aggregates of ∼150 350���KDa (as estimated by Fergusson plot analysis) displayed specific binding to DR4 and, to a lower extent, DR5, in agreement with the immunofluorescence data.
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A nucleus surrounded by solvate shell contains high-molecular mass components of different natures.
However, 8 other classes of toxins were present, including C-type lectins, natriuretic peptide precursors and even high-molecular mass components such as metalloproteases and L-amino acid oxidases.
In both cases (normal and reversed phases), a large fraction of higher-molecular-mass components had retention times similar to those of benzene and coronene.
The paper did not mention any problems related to peak overlap of matrix components and the analyte or elution of high-molecular-mass components (the final distillation temperature of lubricating oil components can exceed 500 °C), which can be expected when using operating conditions described in the paper (final GC oven temperature below 290 °C) [ 13].
In addition, the use of low molecular mass blend components leads to PP-EPDM phase graft-links as well as increased values of elongation and tensile strength at break.
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