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Cleaved Spitz was immuno-captured with anti-Flag agarose and subjected to molecular mass analysis by MALDI-TOF mass spectrometry.
Cleaved N-terminal Flag-tagged Spitz was immuno-captured with anti-Flag agarose and subjected to molecular mass analysis by MALDI-TOF mass spectrometry.
The molecular mass analysis of the culture supernatant produced by the B.subtilis strain SLC using feathers as substrate revealed that most peptides, derived from chicken feathers, presented a molecular mass in the range of 500-3000 Daltons [ 16].
Molecular mass analysis of the native and synthetic peptides and LC-MS analysis of crude C. textile venom were performed on a C18 Phenomenex column (2.1 × 150 mm, 5 μm) eluted with 0 to 60% B in 60 min (A=0.05% TFA; B=0.045% TFA, 90% ACN).
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In the past, the LTP probe, which was first described by Harper et al., was used successfully for direct molecular mass spectrometric analysis with minimal sample pretreatment in a variety of applications.
Molecular mass spectrometry (MS) analysis of protein phosphorylation is partially limited by the molecular specie specificity of the analytical responses that might impair both qualitative and quantitative performances.
The peptide obtained by affinity chromatography was purified by RP-HPLC and used to determine molecular mass by MALDI-TOF analysis.
Furthermore, measurements of molecular mass (gel permeation chromatography analysis) and Carr Index (flowability test) on PHB samples were done before and after the drying.
Major advantages of TEM over other structure determination techniques include minimization of artifacts derived from harsh crystallization conditions, direct visualization of proteins in near-physiological environment, and relatively larger molecular mass limit for the analysis (van Heel et al. 2000).
Transient overexpression of these mutants in HeLa cells demonstrated that only glycomutants at positions N118 and N136 show characteristic CD95 band shifts to the lower molecular mass upon Western Blot analysis (Figure 4B).
To determine the molecular mass of PfaE3, SE analysis was undertaken.
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