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Using genetic interaction and molecular markers, we show that most of these phenotypes are caused by increased activity of the Drosophila EGFR.
Between different molecular markers we found high tumor Ang-4 and Ang-1 expression to be moderately correlated (r = 0.18, P = 0.001).
In order to test the efficiency of molecular markers, we collected 12 morphological species of fig wasps from Ficus benjamina, of which six had polymorphic males and seven were extremely sexually dimorphic.
Considering the 10 selected genes as representative prognostic molecular markers, we tested whether expression of the genes or their subsets could predict the duration of survival in an independent cohort.
In order to follow the neural crest cells in the first branchial arch other than with molecular markers, we replaced the cephalic neural folds (diencephalon, mesencephalon, anterior rhombencephalon) from chick embryos at 5 6 somite-stages, just before neural tube closure, with the quail equivalent (Figure 4A).
Among various molecular markers, we chose COI, Cytb and 28S.
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To the simplicity of the study of a single molecular marker, we may be able to integrate the complexity of multiple biological determinants, participating in signalling pathways and biological networks.
In our study we were able to collect Guthrie cards from 13 AML children with a suitable molecular marker – we found intronic breakpoints in 12 patients with 6 different fusion genes and, moreover, we used for the first time FLT3/ITD for the analysis in two patients.
We next determined whether PIM kinase expression levels might correlate with other molecular markers that we have previously assessed using the same DLBCL cohort (Tzankov et al, 2006, 2008; Meier et al, 2009; Nagel et al, 2010).
The molecular markers that we used had two haplotypes (alleles) at each of the two gene loci, mitochondrial intermediate peptidase (MIP), which is closely linked to mating type locus A in other studied mushrooms (James et al. 2004), and pheromone receptor (**RCB), which is a region within mating type locus B in other mushrooms (Aimi et al. 2005; Brown and Casselton 2001).
Traditionally biomarkers are cell surface or intracellular molecular markers – here we suggest that the degree of leakiness of tumor blood vessels 'integrates' underlying tumor microenvironmental factors related to angiogenesis, and represents the underlying tumor pathomorphological status.
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