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The current study aims to identify molecular markers based on the nuclear ribosomal DNA – ITS1 region to distinguish the authentic species from substitute/adulterants.
In this study we developed strain-specific molecular markers based on polymerase chain reaction amplification of sequence-characterized amplified regions (SCAR) in combination with dilution plating on semi-selective medium to detect and estimate density of propagules of a commercial strain of B. bassiana (strain GHA) in field samples.
Furthermore, we develop molecular markers based on the SNPs found.
This is the first report to our knowledge of the use of GeSNP to identify SFPs within closely related grass species and the development of molecular markers based on validated SFPs.
Another major benefit from these genomes includes the ability to develop molecular markers based on GAS mobile elements to tag and track field-level diversity of the circulating strains; this will be of paramount significance in vaccine development and testing.
The use of molecular markers based on isozymes, RFLPs (restriction fragment length polymorphisms), SSRs (simple sequence repeats) and CAPS (cleaved amplified polymorphic sequences) genetic markers allowed for the construction of early genetic maps.
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Marker-assisted selection is a molecular breeding process whereby a molecular marker based on DNA variation is used for indirect selection of an interest agronomic trait.
Tissue culture, transformation and molecular marker based studies were conducted to improve the plant material.
This property is of vital importance, especially for quantitative assessment of the molecular marker based on the imaging results.
These better resolved mitochondrial and nuclear phylogenetic analyses clearly show many incongruent phylogenetic positions between two types of molecular marker based phylogenies in the northern swordtails.
Two of the lines were subsequently used in the first molecular marker based genetic linkage map of white clover [ 4], which was based on an F2 population of a cross between a fourth generation derivative of one line and a fifth generation derivative of another (population F2(I.4RxI.5 J)).
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