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c Molecular marker genotypes and phenotypes of recombinants.
Since all plants constituting the bulks were chosen to be homozygous in the target interval as determined by the molecular marker genotypes these two SNPs could not be assigned to the target interval on LG1.
Implementation of genomic selection (GS) [ 1] in breeding programs requires dense molecular marker genotypes since increasing marker density increases the probability that a marker is in strong linkage disequilibrium (LD) with a quantitative trait locus (QTL) [ 2].
In modern breeding, introgression of genes controlling useful traits from wild into cultivated species is often facilitated by first associating molecular marker genotypes with trait phenotypes, a process known as gene mapping or quantitative trait locus (QTL) mapping (Collard et al. 2005).
Another explanation is that IG127015 became a wax producer through a de novo recessive mutation at the Iw2 locus; this scenario, however, is unlikely because the molecular marker genotypes in the Iw2 chromosomal region of IG127015 were largely identical to those in the Iw2 chromosomal region of glaucous TauL2 accessions (Table 3).
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For traditional QTL mapping, molecular marker genotyping was time consuming and labor-intensive (Chen et al. 2014a).
Correlations between geographical origin or phenotypic characteristics and the genetic similarity in terms of molecular marker genotype were investigated.
To generate high-density genetic maps, automated high-throughput, low-cost new technologies for molecular marker genotyping are required.
Until recently, molecular marker genotyping techniques which could attend the high demands of sample characterization in germplasm banks were time and resource consuming [ 14].
The flexibility of our genome panel system should improve the efficiency of molecular marker genotyping by reducing the labour intensity and time required for this process, although this system requires a fluorescent sequencer and hundreds of custom-labelled primers with fluorescent dye.
Furthermore, we found that, on the basis of the molecular-marker genotypes in the Iw2 chromosomal region and the phenotypes of the synthetic common wheat lines, virtually all non-glaucous accessions had the Iw2Iw2 genotype (Table 3, Additional file 2).
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