Finally, given that a major goal of our study was molecular marker development, identification of the retrotransposon-derived reads is crucial to avoid developing and genotyping molecular markers residing in such sequences.
More than two million simple sequence repeats were identified, which will facilitate molecular marker development.
The rapid accumulation of rice genome resequencing data not only assisted in the identification of functional genes or QTLs but also provided numerous polymorphic genome sequences for molecular marker development (Huang et al. 2010, 2011, 2012; Xu et al. 2011).
The sequences with potential bacterial, viral, and artificial origins were removed from further annotation and molecular marker development.
EST libraries are also very useful for molecular marker development in linkage mapping, comparative genome analysis, and estimation of gene duplication [16].
Yellow mustard has lagged far behind oilseed Brassica species in molecular marker development and application.
High throughput transcriptome sequencing provides plenty of SSR loci for molecular marker development.
Next generation transcriptome sequencing produces plenty of sequences for molecular marker development.
It has lagged behind oilseed Brassica species in molecular marker development and application.
The 39,257 EST-SSRs predicted in this study laid a solid foundation for molecular marker development in rubber tree.
