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In the cerebellum we investigated the Stratum ganglionare (Purkinje cells), Stratum granulosum (granule cells), Laminae medullares and Stratum moleculare (molecular layer) (Figure 4).
The complete set of morphologies, including the neuronal shape, was detectable exclusively within the molecular layer (Figure 1R).
The expression of INPP4A was uniform across the cerebellum and restricted to the Purkinje cell soma and their dendrites in the molecular layer (Figure 4b,d).
At P5 and P7 in the mutant, Purkinje cells appear distressed with rounded soma and multiple atrophic dendrites extending into the developing molecular layer (Figure 5b,c).
Some have irregularly shaped somas with very short dendrites parallel to the granule cell layer (Figure 6g, arrows), some have small somas and short dendrites terminating in the inner molecular layer (Figure 6g arrowheads), but the majority have large somas with long primary dendrites and several branches projecting to the outer edge of the molecular layer (Figure 6d,g,h).
Some had migrated ectopically into the molecular layer (Figure 1A), where the dendrites of the granular cells reside, suggesting that the cells were unable to respond to local signals regarding positioning.
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Their dendrites radiated into the molecular layer (Fig. 2B).
As seen in high-magnification images, the axons formed large swellings that were also seen in the molecular layer (ML; Figure 2A3,B3).
As shown in Figure 1B and C, Lac-Z staining revealed that blue stain was only observed in the cerebellar cortex and was restricted to granule cells including soma (granular layer), dendrites, and parallel fibers throughout the molecular layer in transgenic (Figure 1B and C), but not wild-type mice (data not shown).
Dendrites extending to the molecular layer could be observed (Figure 2C).
About one half of these cells are neuronal progenitors that are mostly localized in the molecular layer (see percentages in Figure 6U).
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