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The focus is on the 5 CNS species that have been most frequently identified as causing bovine IMI using reliable molecular identification methods (Staphylococcus chromogenes, Staphylococcus simulans, Staphylococcus haemolyticus, Staphylococcus xylosus, and Staphylococcus epidermidis).
The present study was designed to determine the taxonomic diversity and metabolic activity of the actinomycetes community, including 13 traditional medicinal plants collected in Sichuan province, China, using multiple approaches such as morphological and molecular identification methods, bioactivity assays, and PCR screening for genes involved in antibiotics biosynthesis.
Molecular identification methods and drug susceptibility testing are essential for rapid diagnosis and for prompt and adequate antimicrobial therapy.
Because species identification based on phenotypic characterization is time-consuming and can produce ambiguous results [ 1- 6], molecular identification methods have taken precedence.
Broad-range molecular identification methods have facilitated the discovery of novel bacterial species and have resulted in a rapid increase in recognized bacterial taxa (28 ).
Comparative genomic hybridization analyses can contribute to this effort by distinguishing unique genus, species and strain-specific genetic targets for molecular identification methods development.
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In order for any increased culture speed to be valuable, a rapid molecular identification method was essential to identify culture growth in 1 day; this also permitted TB identification in bacterially-contaminated cultures (data not shown).
Therefore, providing a rapid molecular identification method is available that takes 1 2 days to perform as in the case of the GenoType® MTBDRplus method and comparable methods such as in house in situ hybridisation methods [34], [38] the overall turn-around time can be as short as 25 days for MGIT vs approximately 60 days for LJ (Figure 2).
AG carried out FAST plaque assay and molecular identification method.
DNA barcoding is a novel molecular identification method that aids in identifying traditional Chinese materia medica using traditional identification techniques.
Because our molecular identification method does not distinguish M. marinum from M. ulcerans, we performed a light exposure test, which identified yellow colony pigmentation typical for M. marinum.
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