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The purpose of the present study was to develop a molecular genotyping method test by using a real time PCR hybridization probe and applying it to the analysis of C1843T mutations of the Sus scrofa RYR1 gene.
Using a molecular genotyping method to assess 15 variable-number-tandem-repeat markers, they found that the infected strain had not only become genotypically distinct from Sterne, but also from all known B. anthracis isolates.
MLST is a molecular genotyping method appropriate for global analysis.
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Objectives: The AcroMetrix HIV-1 Resistance Proficiency Program was designed to assess proficiency in the identification of HIV-1 drug resistance mutations using molecular genotyping methods.
Blood smears and molecular genotyping methods to distinguish recrudescences from new infections were performed as previously described [12].
Greater diversity among the strains has been revealed by using molecular genotyping methods.
Three molecular genotyping methods based on IS 6110-RFLP, spoligotyping, and VNTR were performed on the 55 MDR/XDR strains.
PCR methods have been used successfully to identify all Brucella species and most of the biovars, offering an improvement over conventional molecular genotyping methods [ 85],[ 86].
The development of molecular genotyping methods offers unique opportunities to obviate these problems and to ascertain the relationship between the presence of individual alleles and disease risk.
Molecular genotyping methods rely on the isolation of bacterial DNA, which is currently not possible from patients with latent TB infection.
We did so by analyzing the MDR/XDR strains by molecular genotyping methods, i.e., insertion sequence 6110 restriction fragment length polymorphism (IS 6110-RFLP), spacer-oligonucleotide genotyping (spoligotyping), and variable number tandem repeats (VNTR).
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