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Finally, we describe a general utility transposon vector for chromosomal engineering and molecular genetics experiments in zebrafish.
This study emerged from the previous, intriguing results of reverse molecular genetics experiments suggesting that the Vibrio protease PrtV confers an "anti-infammatory", seemingly protective effect on human intestinal epithelium while it exerts a pathogenic effect in the intestine of the nematode C. elegans [10].
Haplotype network analysis also showed torsos as well as recurrent mutations, both of which pointed to a high frequency of recombination (Figure 5A), and the occurrence of hotspot recombination was confirmed by molecular genetics experiments based on haplotyping and sperm-typing (Figure 3).
YWB, LH and ZY carried out the molecular genetics experiments.
AB participated in the design of the molecular genetics experiments.
MAF harvested the tissue, performed all molecular genetics experiments and co-drafted the manuscript.
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The Reddy Lab uses an interdisciplinary combination of high-throughput genomics, computational biology, and traditional molecular biology and genetics experiments.
TK carried out the experimental design, the molecular genetics and biochemical experiments.
Additional in-depth molecular genetics studies and experiments are necessary to elucidate the possible biological functions of the truncated MAT1-1-1 gene.
Most of the data accumulated in these databases have been derived from classical molecular genetics wet-laboratory experiments of the pre-genomic era and extracted from peer-reviewed papers by manual curation.
JY participated in the design of the study and carried out the molecular genetics studies, gene transfection experiment, western blot analysis and animal experiments; and drafted the manuscript.
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