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The task-specific term list for biological processes, cellular components, and molecular functions was compiled with the following process.
A diversity of molecular functions was found.
The overrepresentation of nine molecular functions was detected (Table 1A): for example, catalytic and structural molecule activity or binding of calcium ions, nucleic acids, lipids, or proteins.
The most significant association among specific molecular functions was with "DNA-binding transcription factor activity" (1.71E-25), known to be frequent among IDPs [ 3, 5, 14, 20].
At higher radiation dosages (2.5 Gy and 5 Gy), negative regulation of molecular functions was activated, cell's response to cytokine stimulus was changed, and genes involved in the cytokine-mediated pathway were altered.
The highest GO term for molecular functions was protein binding, under 'ligand binding and carrier' categories, which had 87 unique sequences accounting for 18% of the total unique sequences matched in this category and 6% of the total unique sequences.
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Functional annotations of selected genes indicate that 26 molecular functions are significantly affected by insulin (Table 1).
The use of the single gene for two or more molecular functions is called gene sharing.
Most of the altered molecular functions were associated to cellular rearrangement (morphology, function, maintenance, and development), therefore suggesting metabolic adaptations (Table 2).
GO Biological Processes, Cellular Compartments and Molecular Functions were returned for 592 genes using the Homo sapiens GO database [32].
The high sequence conservation suggests their molecular functions are similar.
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