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Because of the apparent similarity in the molecular functions represented in these pathways we reasoned that these may be closely connected within the KEGG pathway map.
The major molecular functions represented are hydrolases, receptors and transferases.
As a result, the diversity of biological processes and molecular functions represented among the sequenced Daphnia genes is relatively modest.
Figures 4 and 5 summarize the relative abundance of biological processes and molecular functions represented in this unigene dataset, according to the Gene Ontology annotation database [ 18].
There was no significant difference (P > 0.05) in the molecular functions represented by genes lying in the GRRs compared with the remainder of the map.
To gain insights into the biological role of the identified prognostic miRNAs, we explored the relationships between their expression and a large compendium of pathways, biological processes and molecular functions represented by gene sets extracted from public (KEGG [ 19], Panther [ 20]) and commercial (GeneGO [ 21] and Ingenuity [ 22]) databases.
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Pathway databases associate an organism's proteins with molecular functions, represent these as reactions, and group the reactions based on shared components: the output of one reaction might be the input, catalyst or regulator of a second, and so on.
The categories "binding" (GO: 0005488) and "catalytic activity" (GO: 0003824) were most common in molecular function, represented by 15,938 and 15,869 transcripts, respectively.
"Molecular Function" represented the largest class of assignments (70,305 contigs/ 166,476 GO counts), followed by "Biological Process" (49,972/108,336) and "Cellular Component" (40,551/65,640).
For the T. dalmanni repeat genes, we then used GeneMerge with Bonferroni adjustment [ 39] to determine if the molecular function represented a nonrandom sample of the putative protein-coding genes in the EST database.
Gene set enrichment analysis with gene ontology (GO) terms revealed sequence-specific DNA binding proteins with transcription factor activity, and sequence-specific DNA binding proteins to be the two groups of most over-represented terms affecting molecular function, representing ~9% of differentially expressed genes (Table 1; see Supplementary Table 1 for the full list).
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