The histological, molecular and functional experiments above have revealed that prenatal exposure to ketamine induces alterations of the cortical GABAergic system of Gad67-GFP adult mice.
Contribution: RA, JML and CR designed research; RA and RB performed molecular and functional experiments; RA, JML, TC, RB, CJ, EM, and CR analyzed and interpreted data; RA designed, performed, and wrote the manuscript; JML and CR supervised the research and critically revised the manuscript.
They are also consistent with molecular-genetic functional experiments implicating serotonin signaling during early cleavage stages on the ventral right side of the embryo (Fukumoto et al., 2003; Fukumoto et al., 2005a; Fukumoto et al., 2005b; Carneiro et al., 2011).
XW carried out most molecular, genetic and functional experiments.
Molecular modelling and functional experiments guided the chemical derivation of the LHCGR agonist and led to the identification of the first TSHR-specific partial agonist Org-41841 [ 27] as well as low-affinity antagonist c52 (compound 52) [ 20].
Ultimately, the reason for such a large number of ORs, GRs, and IRs in C. stygia is unknown and additional molecular biology and functional experiments are required in order to confirm the expression and role of these genes.
Rapid evolution of female-derived endopeptidases and male-derived endopeptidase inhibitors is consistent with a model of sexual conflict between these two molecular classes [ 93, 94], though additional functional experiments are required to evaluate this hypothesis further.
Much of our knowledge on the molecular functional pathways of human leukaemia is derived from experiments with cell lines rather than from work on clinical samples (Sandberg & Ernberg, 2005).
Thus, functional experiments are essential to separate direct (or indirect) molecular interactions from other forms of coordination that may involve other regulatory mechanisms.
