The GO annotations were further refined into biological processes, cellular components and molecular functional annotations.
In addition, all RBPs were classified into three major functional groups according to their molecular functional annotations provided in NCBI: (i) splicing factors, (ii) 3′ UTR/poly(A) binding factors and (iii) microRNA binding factors.
We conclude, on the basis of our molecular and functional annotation data, that CPE failure in AD exists.
At the level of protein class or Gene Ontology (GO) molecular function annotation, we only have 13,591 genes with any experimentally based functional annotations and, of the 8600 GO molecular function terms, most have very few associated gene products.
The mapping of gene ontology functional annotation terms onto the non-redundant full length sequences resulted in 901 of our feline cDNA sequences becoming associated with 647 unique gene ontology molecular function annotation terms resulting in 3219 annotation-gene relationships.
Overall, for more than 85% of the T-box regulated transporters the functional annotation (molecular function and/or biological role) could be improved as compared to the entries in the reference database of NCBI.
In immunology, for example, a newly sequenced molecular sequence without functional annotation is a data point, information is gained by annotating the sequence to answer questions such as which viral strain it originates from, knowledge may be obtained by identifying immune epitopes in the viral sequence, and the design of a peptide-based vaccine using the epitopes represents the wisdom level.
We have used GO molecular function annotations of the proteins linked to each fragment for functional descriptions of fragment clusters (Figure 1E).
When the less reliable electronically inferred functional annotations were excluded, 30% of the ArboreaSet contigs were assigned molecular function annotations.
