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In three GO categories—"biological process," "cellular components," and "molecular function"—we selected the five GO terms with the top P-value scores and then graphed them by both number of transcripts and fold enrichment (Additional file 4: Figure S2b).
For the classes of cellular component, biological process, and molecular function, we extracted instances from the corresponding namespaces of the GO.
For every possible combination of GO biological process and molecular function, we found the number of genes that were involved in both GO terms.
By analysing the proteins on the GO level 2 in the category molecular function we received a total of 9 subgroups as shown in Figure 7, upper middle chart.
We did not test the localisation of these proteins but considering their molecular function, we expect that they will reside in the ER and Golgi membranes and some of them are predicted to be transmembrane ER proteins (Ost48 and l(2)not) (http://flybase.org/).org/
To gain insight into FRMD7 molecular function, we used an IP-MS approach and identified the multi-domain plasma membrane scaffolding protein, CASK, as a FRMD7 interactor.
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To investigate whether the organ marker gene products are associated with specific biological processes, cellular components or molecular functions, we summarized GO annotation available for 16 nodule, 68 root, 75 leaf, 28 stem and 329 flower markers (Table 2).
To detect molecular functions, we use the concepts presented by the Gene Ontology.
For molecular functions, we found enrichment of terms involved in transcription, such as transcription factor activity (GO 0003700, P = 0.0019) and sequence-specific DNA binding (GO 0043565, P = 0.00015).
In order to annotate genes to biological processes and molecular functions we used the DAVID database (based on the GO annotation) [ 55].
With respect to molecular functions, we found a significant (p<0.01) enrichment of genes assigned 'glutathione transferase activity' and 'UDP-glucosyl transferase activity'.
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