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The small number of upregulated genes with an unknown molecular function suggests that miRNAs regulate genes that have a noticeable mutant phenotype and thus have a high chance of experimental characterization and discovery.
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The analysis of molecular function suggested that most of the differentially expressed proteins have a role in binding (69.61 %) and catalytic activity (11.94 %) (Fig. 4).
In addition, gene expression related to binding and catalytic activities was obviously higher than other GO terms in terms of molecular function, suggesting that genes involved in these two GO terms were expressed actively before adolescence.
GO analysis for molecular function suggested that this gene group contained a significantly higher percentage of genes encoding transcription factors (24.6%) (Table 1), while GO analysis for biological process found a higher percentage of genes in the transcription category.
The dependence on molecular oxygen for β-cell function suggests a potential role for hypoxia in type 2 diabetes.
First, despite having only two passing genes, the SHE downregulated category had 19 GO- Molecular Function terms suggesting broad pleiotropic impacts by these two passing genes.
Our scoring function suggests otherwise.
Analysis of the molecular and cellular functions suggests that these proteins are involved in the carbohydrate metabolism, cell to cell signaling (THBS1 and GRB2) and cellular assembly and organization (nuclear GSN, CAP, THBS1, MYO1, TPM, and cytoplasmic GRB2, TBK).
High representations of these biological processes and molecular functions further suggested active and efficient adaptation by horse gram to the external stimuli.
In summary, several of the top-ranked differentially methylated genes in the present study have molecular functions that suggest they may play a role in PCa.
Compared to Douglas-fir, many more Arabidopsis sequences fell into the "Unknown cellular components" and "Unknown molecular functions" classes, suggesting that these GO slim classes contain Arabidopsis genes that are more likely to be absent from Douglas-fir or highly diverged (i.e., resulting in no GO slim assignment for Douglas-fir).
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