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GO analysis of the transcriptome revealed 12 cellular component subcategories, 16 molecular function subcategories, and 42 biological process subcategories (P value <0.05) (Additional file 1: Table S2).
The molecular function subcategories that were most notably differentially expressed between atrial and ventricular muscles included enzyme, signal transducer, structural protein, and transport (see Figure 2).
Gene ontology (GO) analysis of the transcriptome revealed 12 cellular component subcategories, 16 molecular function subcategories, and 42 biological process subcategories (P value <0.05).
aGenes separated into molecular function subcategories represented in the list of gene changing at least 1.45-fold by TNFα as either mitogen-activated kinase kinase (MEK /extracellular regulated kinase (ERK) dependent or MEK/ERK independent.
Compared with the GO annotation results of the DGE screening of genes in the transcriptome data, we found no genes distributed in the three molecular function subcategories (enzyme regulator activity, structural molecular activity, or translation regulator activity) in the DGE group, indicating that these three subcategories are not related to tumour swelling.
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However, the Molecular Function subcategory of "morphogen activity" was only assigned to sequences from E. lineata.
Major cellular components and molecular functions subcategories of protein products from the list of genes were identified.
We organized all Gene Ontology [ 36] annotations into Biological Process (BP), Cellular Component (CC), and Molecular Function (MF) subcategories (Affymetrix, Santa Clara CA).
Sequences with GO terms corresponding to the "cellular component" group fell into 14 subcategories, "molecular function" into 16 subcategories, and "biological process" into 31 subcategories.
The unigenes for the GO analysis were divided into three categories and 53 subcategories: 29.18% unigenes for cellular components (16 subcategories), 14.76% for molecular functions (15 subcategories), and 56.05% for biological processes (22 subcategories).
Similar distribution pattern of GO classification was distinguished among three tissues, with the majority of the GO terms being assigned to biological process distributed in 21 subcategories (∼49%), followed by cellular component in eight subcategories (∼31%) and molecular function in 12 subcategories (∼20%).
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