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All annotation information is presented in Table S1, but only the GO-Slim annotations were used in the analyses described below, specifically focusing on 33 functional categories at the Cellular Component level, 39 categories at the Molecular Function level and 49 categories at the Biological Process level.
Gene ontology was generated by GO Slim with Biological Process and Molecular Function level 2 and Cellular Component level 6.
Combined graphs of the entire transcriptome were constructed for Biological Process and Molecular Function (level 2).
GO molecular function level 5 categories statistically enriched (p-value < 0.01) by HHP for ENV, NEF, and TAT.
Ontological analyses were performed at Molecular Function Level 1, Biological Process Level 2, and Cell Component Level 4 [ 35].
At the molecular function level, there were significantly more DEGs in catalytic activity and binding GO terms than in other terms.
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Thus, a total of 144 sequences were annotated, 132 of them for molecular function and 101 sequences with annotation for molecular function levels 2 or 3. Most of the mapped SNPs were associated to cDNA belonging to the GO terms: binding, catalytic activity and hydrolase activity (Additional file 6).
Enrichment analysis was performed for annotation clustering by KEGG pathway, Gene Ontology (GO) Molecular Function Levels 2 and 3, GO Cellular Component Levels 3 and 4, or GO Biological Process Levels 3 and 4. The threshold for labeling an annotation cluster as enriched was an enrichment score > 1.5.
In addition, PTN, Fibroblast growth factor-10 (FGF10), and serpin peptidase inhibitor, clade E (SERPINE2) also known as glial-derived neurite promoting factor, were classified as heparin binding factors by gene ontology in molecular function at level six.
For each protein its biological process, cellular compartment and molecular function at level two and three of details are used as features.
The whole genome molecular function assignment level in Gene Ontology revealed a predominance of binding genes (80.51%), suggesting these are representatively higher in P. patens genome.
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