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Although many transcripts were encoded unknown function, we still found significant enrichment of GO terms in molecular function including binding, metabolism and transport.
The latter covers a broad spectrum of protein molecular function, including ligand, metal, inorganic cluster, protein, and nucleic acid binding [ 17].
Furthermore, these methods consider many aspects of protein molecular function including binding to small organic molecules, inorganic groups, for example, iron-sulfur clusters and metal ions, and interactions with nucleic acids and other proteins [ 17].
For the abundance-decreased genes, GO annotation reveals their involvement in several subcategories of molecular function including binding (HMOX1, DBPHT1), catalytic activity (HMOX1) and molecular transducer activity (HMOX1) and no assignment for two remaining genes (EEF, EEF2L).
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Molecular Function includes the most genes and is usually based on sequence similarity.
For the up-regulated cluster of differentially expressed ESTs, some of the enriched GO terms for molecular function included ATP binding, actin binding, serine/threonine kinase activity, structural constituent of cuticle, etc.
Similar UVR exposure can also affect key molecular functions including collagen fibril assembly and protease susceptibility [65, 68, 69].
The methylation PSF includes 48 methylated sites in 44 genes coding for basic molecular functions, including transcription regulation, DNA binding, cytokine, and transferase activity.
Gene Ontology (GO) analysis using GoSurfer revealed major categories for genes significantly altered by cocaine, which demonstrated changes in gene expression related to specific molecular functions, including angiogenesis, apoptosis and cell death, cell adhesion, neurogenesis and axon guidance.
These include proteins that are involved in a variety of different molecular functions including vesicle trafficking, purine metabolism, transcription/chromatin regulation, cytoskeletal function, and RNA metabolism.
Increase of the nuclear protein content by heat consequently affects several molecular functions (including DNA-synthesis and -repair) when a certain thermal dose is exceeded.
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