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We also analyzed DEGs based on their molecular function, as well as their cellular component (Additional file 2: Figures S2-S3; Additional file 1: Tables S6-S7).
Whereas, in Chickpea transcriptome, genes are predominantly involved in the protein metabolism of biological process and the transferase activity of molecular function, as well as the chloroplast of cellular component, which indicated notable differences between animals and plants.
In S. scrofa transcriptome, as discovered by this study, the predominant gene clusters are involved in the cellular process and metabolic process of biological process, the binding and catalytic activity of molecular function, as well as the cell, cell part and organelle of a cellular component.
However, many non-homologous proteins share molecular function as a result of convergent evolution, [91] and variations can produce enzymes with similar function but differing sequence motifs [92].
The original GO description defines its molecular function as a generic "GTP binding" property but the subunit was correctly assigned the more specific guanyl-nucleotide release factor activity by ARGOT, PFP and Blast2GO.
In addition to its molecular function as a histone methyltransferase, Huang et al. recently proposed a distinct role for Smyd2 as a putative oncogene by methylating p53 and thereby repressing its tumor suppressive function [33].
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We have also implemented this for molecular functions as well.
Upon interrogation of the internal database, the software converted protein entries into a list of GO terms indicating protein biological and molecular functions, as well as physiological cell localization, using the corresponding gene-GO association table.
GO slim analysis identified broad terms describing biological processes, molecular functions, as well as cellular components to which they belong.
An enrichment of terms detailing specific molecular functions, as well as associations with cellular components were identified within gene sets.
Molecular functions as well as biological processes were compared for genes targeted by both conserved and non-conserved miRNAs to detect any bias in biological function.
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