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The largest portions of ESTs were annotated with the GO Biological Process Annotations and Molecular Function Annotations for metabolic (32% and 21% respectively) and cellular (31% and 20% respectively) processes mostly localized intracellularly (19%) within intracellular particles (17%) or organelles (16%).
We have used GO molecular function annotations of the proteins linked to each fragment for functional descriptions of fragment clusters (Figure 1E).
When the less reliable electronically inferred functional annotations were excluded, 30% of the ArboreaSet contigs were assigned molecular function annotations.
Of 866 proteins, 531 had Molecular Function annotations and 587 had Biological Process annotations.
The output is the summary of the mode-of-action analysis of the corresponding natural medicines, which provides molecular function annotations of target proteins (e.g., molecular functions in Gene Ontology19) and biological pathway annotations (e.g., biological pathways in KEGG PATHWAY12).
The orthologs are accessible through an intuitive web interface that allows searches by genome or gene identifier, presenting results as phylogenetic profiles together with gene and molecular function annotations.
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As for GO molecular function annotation, CA7, IL6R, and SPP1 are annotated as having gelatinase activity; AQP8, SPIB, and TCN1 are annotated as being capable of binding; GUCA2B is annotated as a regulator of enzyme activity, and CWH43 is not annotated.
At the level of protein class or Gene Ontology (GO) molecular function annotation, we only have 13,591 genes with any experimentally based functional annotations and, of the 8600 GO molecular function terms, most have very few associated gene products.
The molecular function annotation for a cluster is obtained by scoring each GO term: (1) based on its frequency of occurrence in that cluster and (2) based on its over-representation in that cluster with respect to overall protein annotations contained in FragKB.
The primary GO molecular function annotation is binding (nucleic acid binding, chromatin binding, and peptide binding).
10% of L. bicolor gene models have EC number annotations and 37% have GO Molecular Function annotation.
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