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Subdivision corresponding to the two molecular forms was revealed by both Bayesian clustering analyses and was also confirmed by PCoA.
In the diagram, the relative position of a karyotype common to both molecular forms was consistent across forms overall.
The coexistence phenomenon of various molecular forms was extensively predicted in many species sequenced that were verified through direct observation using electron microscopy in tobacco [ 33].
Species identification and determination of An. gambiae molecular forms was carried out by PCR as described in Fanello et al. [ 36].
For this purpose, we tested populations of the M and S forms from localities situated on a transect spanning a gradient of urbanization, where habitat segregation between the two molecular forms was originally recorded [ 18].
Divergence between species and genetic differentiation between A. gambiae molecular forms was assessed by sequence-based F statistics (Fst) analogous to Wright F statistics [ 94], calculated according to Hudson et al [ 95].
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Five vascular endothelial growth factor molecular forms were identified with vascular endothelial growth factor164 being the most abundant form expressed.
When K is 5, the M and S molecular forms were further separated (Figure 2A).
The M and S molecular forms are sympatric in seven of the ten localities selected for this study (Table 1).
The levels and pattern of the molecular forms are similar to that observed in individuals with silent BuChE.
The probability of the G119S mutation to have been gained twice independently at the same position on a same susceptible allele in both molecular forms is extremely low.
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