DNA samples were diluted to 50-100 ng/uL and then quantified in duplicate by fluorometry using PicoGreen™(Molecular Probes, Eugene, OR, US).
The quantity recovered was determined by fluorometry using SYBR Green II (Molecular Probes, Leiden, The Netherlands).
DNA was quantified for quality control by fluorometry using PicoGreen (Invitrogen).
The N-terminal SMN antibody, followed by a secondary antibody conjugated to Cy3 (GE Healthcare, 1∶4000) was used for quantification of calpain cleavage by fluorometry using unsaturated digital scans performed on a Typhoon Trio+ Variable Mode Imager (GE Healthcare).
Detection of residual DNA was performed with the PicoGreen® fluorescent dye assay (Invitrogen Canada, Burlington, ON) and measured by fluorometry using Lambda DNA for the standard curve (Invitrogen Canada, Burlington, ON).
Here a new system to assay cell Aβ aggregation was developed by combinations of microglial culture with immunofluorescence and fluorometry using 96-well tissue culture plates (see Materials and Methods).
The analysis was performed by fluorometry using freeze dried, dissected muscle specimens as described [ 36].
for fluorometry using a fluorescence plate leader (Polarion; Tecan Japan Co., Ltd).
Two common methods for DNA quantification are spectrophotometry and fluorometry using DNA-binding dyes.
The total RNA amount was measured by fluorometry using a Qubit™ - Quant-iT™ RNA Assay Kit (Invitrogen, USA).
