Assumptions that explain the relation between complement activation mechanisms triggered by the nanoparticles and the nanoparticle corona molecular feature were proposed.
The mass cytometer tallies how many copies of each metal-tagged antibody were stuck to the cell and, therefore, how many copies of each molecular feature were present on, or in, the cell in the first place.
Although the target tissues and symptoms are markedly different among these diseases, a common molecular feature is the increased expression of interferon-regulated genes [ 7].
This molecular feature is indicative of the retro-positioning which occurs when an mRNA is retro-transcribed to cDNA and then inserted into the genome.
The median overall precision of all 5,891 molecular features was 18.3 % RSD.
As a first step, the effect of the production system and the production year on each of the 5,891 variables (molecular features) was investigated by two-way ANOVA.
Motif search for common molecular features was conducted through MotifSearch.Com, an online motif search tool built on the state-of-the-art qPMS7 algorithm (Dinh et al. 2012).
The correlation of MACC1 expression with clinicopathological and molecular features was evaluated using the Chi-Square, or Fisher's Exact test as appropriate.
The molecular subtype of each of the 101 HCA (see Additional file 1 for clinical and molecular features) was determined according to an established molecular classification using gene mutation and immunohistochemistry staining [ 4- 6].
A summary of baseline clinical and molecular features is provided, Supplementary Table 1.
