IT and MN carried out the molecular genetics experiments, IT and GW performed laser microdissection, IT carried out fluorescence microscopy, MN performed the bioinformatics analyses, MN drafted the manuscript, IT and UK helped draft the manuscript, and MN and UK designed the study.
Prior to beginning complex downstream molecular experiments on the LMD samples it is crucial to perform a pilot study for optimisation of the sample processing and LMD with the aim to consistently achieve RNA samples of adequate quality and quantity for successful downstream analysis and to evaluate feasibility, reliability and cost effectiveness of the procedure.
Furthermore, it is worth doing more molecular experiments on the potential invasive and metastatic suppressor function of GalNAc-T6 in pancreatic cancer.
DMB participated in its design and coordination, performed molecular experiments and wrote the manuscript.
designed the molecular experiments, and Z.Y. and G.H. performed the molecular experiments and led interpretation of the molecular-data analysis.
Z.L.Y. and B.F. designed the experiment; B.F., X.-H.W., D.R., G.G., S.S.L. and T.G. collected the samples; B.F. conducted molecular experiments; B.F. and Z.L.Y. analyzed the data and drafted the manuscript; all authors improved the manuscript.
We would like to thank M. Kimura, K. Oba, and M. Mawatari for support in cell biological and molecular experiments, N. Shinohara for constructing pFlex-mRFP vector and members of the ERATO Reprogramming Evolution Project for technical assistance.
For identification of hau and gho (PCR & sequencing), molecular experiments were performed following standard protocols for molecular biology.
Even though both experiments are quite different (molecular experiments vs. in vivo electrophysiological recordings), yet this coincidence is rather intriguing.
MM performed molecular experiments.
