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The molecular estimates are older than the corresponding archaeological dates.
Beyond this clade, no two molecular estimates are identical with respect to the remaining species.
Through analyses of a large mtDNA matrix using multiple cladistically tested calibrations, alterative tree topologies and several sophisticated relaxed clock methods we have found that the molecular estimates are robust to varying assumptions about the evolution of evolutionary rates and consistent with those from previous studies.
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This molecular estimate is intriguingly close to the earliest fossil beaver remains in North America possibly dating as early as 7.5 mya [12].
We demonstrate that even with identical calibration regimes and molecular clock methods, mitochondrial based molecular age estimates are systematically older than those estimated from nuclear sequences.
But these "molecular clock" estimates are notoriously unreliable.
Since such molecular clock estimates are subject to some uncertainty we also made an extremely conservative dating by assuming that all mutations accumulate neutrally in genomes, i.e. in the absence of purifying selection.
This study further illustrates that, just as paleontological dating, molecular dating estimates are often associated with large uncertainties.
These molecular time estimates are compatible with time estimates (2.6 2.7 Ga) based on geological evidence for the earliest colonization of land by organisms (prokaryotes) [ 85].
This is an interesting result given that molecular rate estimates are very often found to be older than expectations from the fossil record [ 71].
To gain further insights into the history of Begonia diversification, molecular divergence time estimates are also performed.
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