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On the other hand, they show that even after two centuries of microscopy studies and about 20 years of molecular diversity analyses, HTS sequencing approaches still reveal substantial amounts of novel diversity.
P. fulvum also forms a distinct clade in all molecular diversity analyses [[ 6] and references therein] and is the only realistic candidate in the genus for a distinct species.
We are currently collecting P. uniformis strains from many additional locations in Europe and North America to study the origin of this species using molecular diversity analyses and relate these studies to similar investigations of the CPB [ 37].
Eighty-nine accessiofs of B. napus, B. juncea, B. rapa and B. carinata comprising contemporary rapeseed cultivars and elite lines from Australian breeding programmes were chosen for molecular diversity analyses along with accessions from China, Germany, Japan, India, France, and Ethiopia (Table 1).
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The genetic structure of the populations and basic parameters of molecular diversity, including analyses of molecular variance (AMOVA) [11], haplotype frequency, haplotype diversity, haplotype sharing and linkage disequilibrium analyses were calculated using the package Arlequin 2.0 [12.0
The molecular genetic diversity analyses revealed several attributes characteristic for all species: (1) Ecotypes from one country were usually more closely related than those from different countries (as in case of Lithuanian ecotypes of F. pratensis 3982 and 3985).
Molecular diversity was analysed for 290 individuals from 12 populations in the Pyrenees and the Cantabrian Mountains using inter simple sequence repeats (ISSRs).
ARLEQUIN version 3.5.1.3 [ 60] was used for the calculation of mismatch distributions, molecular diversity indices, and analyses of molecular variance (AMOVA) with entire control region sequences (16024 576) excluding C-insertions on positions 16193, 309, 315 and 573.
Analyses of molecular diversity indicate that most L. boeticus individuals in extant populations are descended from one or two relatively recent population bottlenecks.
Molecular diversity of RT sequences was analysed using polymerase chain reaction and denaturing gradient gel electrophoresis (DGGE).
Here, we have analysed the molecular diversity and genetic structure of this collection, using simple sequence repeats (SSRs), while taking into account not only the environmental adaptation (geographical and altitude factors), but also, for the first time, the two growing seasons per year.
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