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We aim to determine the applicability of molecular diagnostic procedures for monitoring chronic osteomyelitis, and to evaluate if these procedures are superior to standard culture methods of osteomyelitis detection.
Periodic health control, with parasitological examination, by sensitive molecular diagnostic procedures (PCR), will be essential.
This procedure would compensate for the slightly lower specificity of the antigen-capture assay and should result in markedly decreased workloads for laboratories using molecular diagnostic procedures.
Because of the significant public health implications, the spread of organisms producing CTX-M-producing isolates merits close monitoring with enhanced surveillance efforts [ 31], with the introduction of molecular diagnostic procedures in a clinical or reference laboratory to track their spread in the community and hospital settings.
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In this study, we addressed the following questions: (1) Is the molecular diagnostic procedure applicable for monitoring chronic osteomyelitis?; and (2) Does molecular diagnostic perform better than culture methods for osteomyelitis detection?
Thus, the purposes of our study were to determine (1) the applicability of the molecular diagnostic procedure for monitoring chronic osteomyelitis, and (2) whether molecular diagnostic is superior to culture methods of osteomyelitis detection.
Two-step molecular diagnostic procedure is used in DM1 genetic testing [ 106].
Therefore this study describes a more expansive molecular diagnostic procedure for avian influenza viral subtyping and sequencing that may help researchers to perform comprehensive approaches on influenza surveillance and virus subtype identification.
The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing.
In contrast, the far less common form, FSHD2, is highly similar to FSHD1 in clinical presentation, yet it is contraction-independent and cannot be diagnosed or excluded by this common molecular diagnostic procedure [ 16, 17].
The use of kit-based reagents will enable a wide range of laboratories to undertake molecular-based diagnostic procedures for RNA viruses and provide results within a time frame relevant to patient management.
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