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Data, filtered at 1 kHz and digitized at 10 kHz, were acquired using an Axopatch 200B amplifier and a Digidata 1320A data acquisition board (Molecular Devices Data) were acquired using pClamp8.02 (Molecular Devices) and analyzed with Clampfit8.02 (Molecular Devices) or MiniAnalysis (Synaptosoft).
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Initial rates of reaction at various concentrations of triclosan were monitored by spectrophotometry (SpectraMax 190, Molecular Devices) and data were analyzed using Excel Microsoftt).
Data were acquired with a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), data acquisition boards (models PCI MIO 16E-4 and 6713, National Instruments, Austin, TX), and custom-modified version of Ephus software (Ephus, available at https://www.ephus.org/).org/
Data were acquired with a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), data acquisition boards (models PCI MIO 16E-4 and 6713; National Instruments, Austin, TX), and custom modified version of Ephus software (Ephus, available at https://openwiki.janelia.org/).org/
The head stage was attached to a digitally controlled micro-manipulator (Luigs & Neumann SM-7) and connected to a multi-clamp 700 amplifier (Molecular Devices): analogue electrophysiological data was low-pass filtered at 6 kHz and subsequently converted to digital values at a sample rate of 20 kHz using a Digidata 1440 A (Molecular Devices).
Images were captured using MetaMorph (Molecular Devices) software and data analysis was carried out with ImageJ (NIH).
At 72 hours post transfection, luciferase activity, which is a marker of cell proliferation, was measured using an Analyst GT Multimode Reader (Molecular Devices) and all data points were plotted as a percentage of control siRNA treated cells.
MenA-specific IgG and IgG subclass concentrations were calculated with a 4-parameter, logistic curve model in the SOFTmax PRO (Molecular Devices, Wokingham, UK) data analysis software program.
Microarrays were scanned at 10-μm resolution using a GenePix Personal 4100A microarray scanner and the GenePix Pro 6.0 acquisition and data-extraction software (Molecular Devices, Corp .. Raw data were processed and analyzed with Acuity 4.0 (Molecular Devices, Corp).
Fluorescence intensity data were recorded using GenePix Pro v6.0 (Molecular Devices) (supporting information, Table S1).
The data was filtered at 2 kHz, and digitized (Digidata 1322A; Molecular Devices) at 10 kHz; data acquisition and analysis was performed with pClamp 9.2 software (Molecular Devices).
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