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The one-step nucleic acid amplification (OSNA) assay (Sysmex Corporation) is a rapid molecular detection procedure that analyses lymph-node metastases by detection and amplification of cytokeratin 19 (CK19) mRNA (Tsujimoto et al, 2007).
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A widely used alternative to immunocytochemical assays for the detection of DTCs comprises molecular detection procedures.
The one-step nucleic acid amplification (OSNA) assay is a molecular-based lymph-node metastasis detection procedure that can assess a whole node and yields semi-quantitative results for the detection of clinically relevant nodal metastases.
Things have changed rapidly on these three fronts, as (1) several theoretical models now make explicit predictions regarding the fitness effects of new mutations, (2) the experimental evolution approach has greatly expanded within evolutionary biology, and (3) the advent of cost-effective high-throughput sequencing makes molecular detection of new mutations in genomes a routine procedure.
Dried blood spot (DBS), a minimally invasive procedure, is an alternative to serum and can be used for field based studies for molecular detection.
Figure 11 Watermark detection procedure.
Different strategies have been developed to simplify the detection procedure, which involve specific metal coordination, epitope-docking on miniature proteins, aptamer selection, non-natural peptide isosteres, functionalized platforms, secondary structure mimetics, molecular imprinting and receptors embedded in lipid layers.
A simple watermark detection procedure is applied.
This portion of DNA forms the basis of molecular detection.
Fig. 7 Flowchart of the proposed tamper detection procedure.
Then, some discussion about the detection procedure is provided.
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